Application

Detect CD133 using this mouse monoclonal Anti-CD133 (Prominin-1) antibody, clone 17A6.1, Cat. No. MAB4399-I. Validated for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CD133 in U251 cells.

General description

Prominin-1 (UniProt: O43490; also known as Antigen AC133, Prominin-like protein 1, CD133) is encoded by the PROM1 (also known as PROML1, MSTP061) gene (Gene ID: 8842) in human. CD133 (Prominin-1) is multi-pass cell-surface glycoprotein that plays a role in cell differentiation, proliferation and apoptosis. It binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development CD133 acts as a key regulator of disk morphogenesis. Defects in PROM1 gene are known to cause retinitis pigmentosa, cone-rod dystrophy, and retinal macular dystrophy 2. Higher levels of CD133 have been reported in several cancer cell types, including various leukemias, retinoblastomas, glioblastomas, and kidney carcinomas. Higher levels of CD133 are also observed in pancreatic, gastric, colorectal, and hepatocellular cancers. It is also considered as a marker of stem cells in the adult small intestine that are susceptible to transformation into tumors retaining a fraction of mutant Prom1+ tumor cells. (Ref.: Zhu, L., et al. (2009). Nature 477 (7229); 603-607).

Immunogen

GST-tagged recombinat fragment corresponding to 77 amino acids from the N-terminal region of human CD133 (Prominin-1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting with recombinant Prominin-1.

Western Blotting Analysis: 1 µg/mL of this antibody detected 2.5 µg of recombinant Prominin-1 protein.

Specificity

Clone 17A6.1 detects recombinant CD133 (Prominin-1) by Western blotting and also detects CD133 (Prominin-1 ) in U251 cells by immunocytochemistry.

Target description

~97 kDa observed; 97.2 kDa calculated. Uncharacterized bands may be observed in some lysate(s).